Composite
Part:BBa_K4385018
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-06)
Lead-inducible surface display
The part mainly consists of the lead-inducible promoter pbr , the anchoring protein Lpp-OmpA and the heavy metal detoxification protein metallothionein. When the lead ion concentration in the environment exceeds a certain concentration, the pbr promoter initiates transcription of downstream OmpC-CBP, witch is used as an anchoring protein to anchor PbrR on the surface of E. coli for lead ion adsorption.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1809
Illegal NheI site found at 1832
Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1944
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI site found at 1360
[edit]
Categories
Parameters
None |